LOCUS       40924_49117        3721 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD021, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3721)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3721)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..536
                     /note="S7942NS2-RA; right arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    445..450
                     /label=SacI
                     /note="SacI"
     rep_origin      573..1161
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1347..1487)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_recomb     1569..2510
                     /note="S7942NS2-LA; left arm of the homologous
                     recombination site NS2 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U44761"
     misc_feature    2511..2516
                     /label=NheI
                     /note="NheI"
     misc_feature    2517..2537
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2535..2540
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(2664..3521)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3522..3626)
                     /label=AmpR promoter
     misc_feature    3652..3657
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagagttgc taccaagtag tgacccgctt cgtgatgcaa aatccgctga
      121 cgatattcgg gcgatcgctg ctgaatgcca tcgagcagta acgtggcacc ccgcccctgc
      181 caagtcaccg catccagact gaacagcacc aagaggctaa aacccaatcc cgccggtagc
      241 agcggagaac tacccagcat tggtcccacc aaagctaatg ccgtcgtggt aaaaatcgcg
      301 atcgccgtca gactcaagcc cagttcgctc atgcttcctc atctaggtca cagtcttcgg
      361 cgatcgcatc gatctgatgc tgcagcaagc gttttccata ccggcgatcg cgccgtcgcc
      421 ctttcgctgc cgtggcccgc ttacgagctc gtttatcgac cacgatcgca tccaaatccg
      481 cgatcgcttc ccagtccggc aattcagtct ggggcgtccg tttcattaat cctgatagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1261 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1321 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     1381 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1441 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     1501 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1561 ctccgcgggc cacgatttga gggacgaatc ttcagcgtga ctgaagtacc tcgcggcaag
     1621 ccatttcccg atgtcttttt gttggccgcc gatcgcttcg gggttaatcc tacggcctgc
     1681 gctgtgatcg aagacacccc cttgggagta gcggcaggcg tggcggcagg aatgcaagtg
     1741 tttggctacg cgggttccat gcccgcttgg cgtctgcaag aagccggtgc ccatctcatt
     1801 tttgacgata tgcgactgct gcccagtctg ctccaatcgt cgccaaaaga taactccaca
     1861 gcattgccca atccctaacc cctgctcgcg ccgcaactac acactaaacc gttcctgcgc
     1921 gatcgctctt actgttgatg gctcgtgctt aaaaacaatg caaccctaac cgtttcagct
     1981 ggtgattttc ggacgatttg gcttacaggg ataactgaga gtcaacagcc tctgtccgtc
     2041 attgcacacc catccatgca ctggggactt gactcatgct gaatcacatt tcccttgtcc
     2101 attgggcgag aggggagggg aatcttctgg actcttcact aagcggcgat cgcaggttct
     2161 tctacccaag cagtggcgat cgcttgattg cagtcttcaa tgctggcctc tgcagccatc
     2221 gccgccacca aagcatcgta ggcgggacgt tgttgctcca gtaaagtctt cgcccgtaac
     2281 aatccccagc gactgcgtaa atccgcttcg gcaggattgc gatcgagttg ccgccacagt
     2341 tgtttccact gggcgcgatc gtcagctccc ccttccacgt tgccgtagac cagttgctct
     2401 gccgctgcac cggccatcaa cacctgacac cactgttcca gcgatcgctg actgagttgc
     2461 ccctgtgcgg cttcggcttc tagcgcagct gcttggaact gcacaccccc gctagcatcg
     2521 cgcgcgcgcg cgcggacgtc ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3661 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3721 c
//
